This purified ABfinity™ Recombinant Rabbit anti-p53 [pS15] monoclonal antibody is specific to human p53 [pS15], and does not recognize non-phosphorylated p53. p53 [pS15] is encoded by the TP53 gene (Gene ID 7157) and is also known as tumor protein p53, p53, LFS1, and TRP53. ABfinity™ recombinant rabbit anti-p53 [pS15] monoclonal antibody is used in flow cytometry, immunocytochemistry, immunofluorescence, and TR-FRET applications.

Antibody Specifications:

• Applications: Validated applications for this monoclonal antibody are flow cytometry, immunofluorescence, and TR-FRET.
• Clone ID of Monoclonal Antibody (mAb): The rabbit anti-p53 [pS15] monoclonal antibody clone is 14H61L24.
• Host Species and Isotype: The host species and isotype of the antibody is rabbit IgG.
• Reactivity: Reacts with human p53 [pS15].
• Product Size: Rabbit anti-p53 [pS15] monoclonal antibody is available in a 100 µg size.

p53 is a 53 kDa protein normally found in the cell nucleus. The protein forms a tetramer which binds to specific DNA sequences where it serves to enhance the transcription rate of certain genes (including p21/waf1, mdm2, cyclin G, bax, and others), or inactivate the transcription of certain other genes (including topoisomerase Ia). p53 controls the cell cycle by regulating the G1 to S transition, preventing DNA replication when the mitotic spindle is damaged, and preventing the G2 to M transition. The level of p53 expression is observed to increase in response to certain stimuli including gamma irradiation-induced DNA breakage and arrest of DNA or RNA synthesis. Overexpression of p53 inhibits entry into mitosis, and therefore serves to prevent replication of damaged cells. Aberrant p53 expression causes a variety of problems; p53 over-expression inhibits TFF2 mRNA, protein, and promoter activity leading to apoptosis, while loss of p53 favors symmetric cell division. p53 protein accumulation is controlled by Polo-like kinase 1 (Plk1) through phosphorylation of topoisomerase I-binding protein (Topors).