This purified rabbit anti-FAK [pY397] monoclonal antibody ABfinity™ recombinant is specific to Human FAK [pY397] and does not recognize non-phosphorylated FAK protein. Anti-FAK (Focal Adhesion Kinase) [pY397] recognizes the expressed product of the PTK2 gene. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Applications that rabbit anti-FAK [pY397] monoclonal antibody ABfinity™ recombinant can be used in are western blotting, ELISA, immunofluorescence, and immunocytochemistry.

Antibody Specifications:

• Applications: Validated applications for rabbit anti-FAK [pY397] monoclonal antibody ABfinity™ recombinant are western blotting, ELISA, immunohistochemistry, and immunofluorescence.
• Host Species and Isotype: The host species and isotype of the FAK [pY397] antibody is rabbit IgG.
• Clone ID of Monoclonal Antibody (mAb): The rabbit anti-FAK [pY397] monoclonal antibody ABfinity™ recombinant clone is 31H5L17.
• Reactivity: Reacts Human FAK [pY397].
• Product Size: Rabbit anti-FAK [pY397] monoclonal antibody ABfinity™ recombinant is available in a 10 mini-blot size.

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Regulatory mechanisms include phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion.