ABfinity™ Recombinant Rabbit Monoclonal Antibody validated to Human and expected reactivity with human, mouse, rat, equine, opossum, bovine, orangutan, chicken, swine, canine, carp, zebrafish, platypus, salmon, Xenopus. This antibody is validated for use in Western Blot, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. AMPKβ1 [pS182] is encoded by the 5564 gene, also known as AMPK, HAMPKb, MGC17785.
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and is a key target for treating Type 2 diabetes and obesity (1-7). AMPK exists as a heterotrimeric complex comprised of a catalytic α subunit (62 kDa) and non-catalytic β and γ subunits. The β subunit has at least three isoforms, designated β1, β2 and β3. AMPK is phosphorylated by upstream kinases, including AMPK Kinase (AMPKK) and LKB1, which results in AMPK activation (8). Activated AMPK in turn regulates metabolism by phosphorylating rate-limiting enzymes such as acetyl-CoA carboxylase and beta-hydroxy beta-methylglutaryl-CoA reductase, which are required for de novo fatty acid biosynthesis. The phosphorylation site recognized by this antibody is S182 of the β1 isoform. Reactivity with other β isoforms has not been determined.
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