IRF2 ABfinity™ Recombinant Rabbit Monoclonal Antibody is validated to react with human and has an expected reactivity with, Mouse, rat and bovine IRF2. This antibody is validated for use in Western Blot and Immunofluorescence. IRF2 is encoded by the 3660 gene, also known as Interferon regulatory factor 2.
IRF2 belongs to the interferon regulatory factor family. IRF2 regulates the transcription regulation important to cell proliferation (1) as well as immune responses by binding to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and repression of those genes (2). However, IRF2 also functions as a transcriptional activator of histone H4 by interactions with NF-kappa B (3).
1. Ichikawa, E., Hida, S., Omatsu, Y., Shimoyama, S., Takahara, K., Miyagawa, S., Inaba, K. and Taki S. (2004) Defective development of splenic and epidermal CD4+ dendritic cells in mice deficient for IFN regulatory factor-2. Proc. Natl Acad. Sci. 101: 3909–3914
2. Harada, H., Fujita, T., Miyamoto, M., Kimura, Y., Maruyama, M., Furia, A., Miyata, T. and Taniguchi, T. (1989). Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes. Cell 58: 729–739.
3. Drew, P.D., Franzoso, G., Carlson, L.M. Biddison, W.E., Siebenlist, U., and Ozato, K. (1996). Interferon regulatory factor-2 physically interacts with NF-kappa B in vitro and inhibits NF-kappa B induction of major histocompatibility class I and beta 2-microglobulin gene expression in transfected human neuroblastoma cells. J. Neuroimmunol. 63: 157–162