This purified ABfinity™ recombinant rabbit anti-CUL2 monoclonal antibody reacts to human, mouse and rat CUL2. Based on sequence identity and similarity, reactivity to primate, canine, and numerous other species is expected. Validated applications for CUL2 monoclonal antibody are Western blotting, flow cytometry, immunofluorescence, and immunohistochemistry.
Applications: Validated applications for this rabbit anti-CUL2 monoclonal antibody are Western blotting, flow cytometry, immunofluorescence, and immunohistochemistry.
Host species and isotype: The host species and isotype of the CUL2 monoclonal antibody is rabbit IgG.
Clone ID of monoclonal antibody (mAb): The rabbit anti-CUL2 monoclonal antibody clone is 50H17L12.
Reactivity: Rabbit anti-CUL2 monoclonal antibody detects human, mouse and rat CUL2. Based on sequence homology resactivity to primate, canine, and numerous other species is expected.
Product size: Rabbit anti-CUL2 monoclonal antibody is available in a 100 µg pack size.
In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1, and one of many F-box proteins. A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identified (cul1, 2, 3, 4a, 4b, and 5). In humans, Cul2 is part of the ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex which contains the Cullin-RBX module. human Cul2 does not associate with Skp1 or Skp2. Instead, it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL. The Cul2-Rbx1 module specifically interacts with the VHL box. Elongin C shows homology with Skp1, and Elongin B with ubiquitin. In its active form, SIII does not contain VHL, but rather contains an F box protein, Elongin A.