Smad2 [pT8] ABfinity™ Recombinant Rabbit Monoclonal Antibody is validated to react with human and has an expected reactivity with bovine, rat, drosophila, xenopus, and mouse. This antibody is validated for use in Western Blot. Smad2 [pT8] is encoded by the 4087 gene, also known as Mad-related protein 2, MADH2 protein, Mad2 protein.
SMAD2, also known as MADH2 or MAD2 regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation (1). Smad2 interacts with the TGF-beta receptors (2, 3) through its interaction with the SMAD anchor for receptor activation (SARA) protein. The regulation of Smad2 phosphorylation on threonine 8 by ERK1 (4-7) and calmodulin is critical for Smad2-mediated signaling. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors and thereby participating in the regulaton of gene expression. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGFβ signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal-regulated kinase 1 (ERK1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin
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