This purified ABfinity™ recombinant rabbit anti-SMAD2 monoclonal antibody is specific to human SMAD2. Based on sequence identity and similarity, reactivity to orangutan, bovine, chicken, Xenopus, opossum, mouse, rat, zebrafinch, carp, zebrafish, and goldfish is expected. SMAD2 is encoded by the 4087 gene, also known as SMAD family member 2, JV18, MADH2, MADR2, JV18-1, hMAD-2, and hSMAD2. ABfinity™ recombinant rabbit anti-SMAD2 monoclonal antibody is used in western blotting, flow cytometry, immunofluorescence, immunocytochemistry, and ELISA applications.
• Applications: Validated applications for rabbit anti-SMAD2 monoclonal antibody are western blotting, ELISA, flow cytometry, and immunofluorescence.
• Host Species and Isotype: The host species and isotype of the antibody is rabbit IgG.
• Clone ID of Monoclonal Antibody (mAb): The rabbit anti-SMAD2 monoclonal antibody clone is 31H15L4.
• Reactivity: Reacts with human SMAD2.
• Product Size: Rabbit anti-SMAD2 monoclonal antibody is available in a 100 µg size.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Members of the Smad family transmit TGF-β signals from the cell surface into the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smads (co-Smads), including Smad4, and the antagonistic or inhibitory Smads (I-Smads), including Smad6 and Smad7. Impaired Smad2 activity has been linked to TGF-β resistance in multiple myeloma through the activation of CDKs. Aberrant activation of CDKs results in phosphorylation of Thr8 and subsequent inactivation of Smad2, preventing Smad2-Smad4 interaction resulting in loss of transcriptional regulation by Smad2.