This purified ABfinity™ recombinant rabbit anti-SMAD1/5 [pS463/465] monoclonal antibody is specific to human SMAD1/5 [pS463/465] and does not recognize non-phosphorylated SMAD1/5. Based on sequence identity and similarity, reactivity to mouse, rat, primates, and numerous other species is expected. SMAD1/5 is also known as SMAD1: BSP1, JV41, JV4-1, MADH1, MADR1 SMAD5: Dwfc, JV5-1, MADH5, DKFZP781C1895, and DKFZP781O1323. This rabbit anti-SMAD1/5 [pS463/465] monoclonal antibody is used in immunocytochemistry, flow cytometry, and immunofluorescence applications.
• Applications: Validated applications for this monoclonal antibody are immunocytochemistry, flow cytometry, and immunofluorescence.
• Host Species and Isotype: The host species and isotype of the antibody is rabbit IgG.
• Clone ID of Monoclonal Antibody (mAb): The rabbit anti-SMAD1/5 [pS463/465] monoclonal antibody clone is 31H14L11.
• Reactivity: Reacts with human SMAD1/5 [pS463/465].
• Product Size: Rabbit anti-SMAF1/5 [pS463/465] monoclonal antibody is available in a 100 µg size.
Smad proteins are intracellular signal transducers and downstream effectors of TGF-β/BMP signaling. Three distinct classes of Smads have been defined: the receptor-regulated (R-Smads), which include Smad1, 2, 3, 5, 8; the common-mediator Smads (co-Smads), including Smad4, and the antagonistic or inhibitory Smads (I-Smads), including Smad6 and 7. Smad1 (MADR1) mediates signals of bone morphogenetic proteins (BMPs) in the cell. BMPs play a role in ectopic bone formation and inductive interactions during the early development. BMPs constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis of both vertebrates and invertebrates. Phosphorylation of Smad1, a human homolog of MAD, is regulated and rapidly induced by bone morphogenic proteins (BMP) but not TGFβ or activin. BMP receptors are members of the TGFβ family of Ser/Thr kinase receptors, and ligand binding induces multimerization, autophosphorylation and activation of these receptors. Subsequently, they phosphorylate Smad1 at Ser463 and Ser465 in the C-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. Signaling through the BMP-Smad 1/5/8 pathway has been implicated in embryonic morphogenesis (8). The dual phosphorylation site recognized by this antibody is S463/S465 in Smad1 (Accession Q15797) and Smad5 (Accession Q99717) and S465/S467 in Smad8 (Accession O15198).