This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes FOXP3.

ABfinity™ FOXP3 antibody advantages include:

•  Superior lot-to-lot consistency
•  Extremely high specificity and sensitivity
•  Recombinant rabbit monoclonal antibody

Specific for FOXP3
FOXP3 (FORKHEAD BOX P3) belongs to the FOX protein family and the master regulator in T-Cell development and function (Ref 1). The gene encodes protein which is a member of the forkhead/winged-helix family of transcriptional regulators (Ref 2, 3). It is shown that in human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage. FOXP3 is selectively expressed by a subset of CD4+ T cells, known as T regulatory (Treg) cells, which suppress effector T-cell function in response to self or foreign antigens. T regulatory (Treg) cells, known to suppress T-cell activity during response to foreign or self antigen selectively express FOXP3 (Ref 4, 5).

Exceptional Consistency Helps Save You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with high consistency between lots, minimizing the need to revalidate your assays for each lot.

High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies permits an extremely high degree of reliability. Staining of non-specific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting (Fig 1).

High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.

Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinityantibodies produces a 150kDa band under non-reducing conditions, and 50kDa and 25kDa bands under reducing conditions.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
•  Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
•  See Invitrogen's full line of ABfinity™ recombinant monoclonal antibodies.

References
•  Jung, D.J., Jin, D.H., Hong, S.W., Kim, J.E., Shin, J.S., Kim, D., Cho, B.J., Hwang, Y.I., Kang, J.S., and Lee, W.J. Foxp3 expression in p53-dependent DNA damage responses. The Journal of biological chemistry 285, 7995-8002.
•  Schubert, L.A., Jeffery, E., Zhang, Y., Ramsdell, F., and Ziegler, S.F. (2001). Scurfin (FOXP3) acts as a repressor of transcription and regulates T-cell activation. The Journal of biological chemistry 276, 37672-37679.
•  Zhang, L., and Zhao, Y. (2007). The regulation of Foxp3 expression in regulatory CD4(+)CD25(+)T cells: multiple pathways on the road. Journal of cellular physiology 211, 590-597.
•  Coffer, P.J., and Burgering, B.M. (2004). Forkhead-box transcription factors and their role in the immune system. Nature reviews 4, 889-899.
•  Curiel, T.J. (2007). Regulatory T-cell development: is Foxp3 the decider? Nature medicine 13, 250-253.