This purified ABfinity™ recombinant rabbit anti-ERK1/2 [pT185/pY187] monoclonal antibody is specific to human ERK1/2 [pT185/pY187], and does not recognize non-phosphorylated ERK1/2. Based on sequence identity and similarity, reactivity to mouse, rat, chimpanzee, bovine, chicken, Xenopus, and zebrafish is expected. ERK1/2 [pT185/pY187] is also known as ERK1: PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, ERK2: ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, and p41mapk. ABfinity™ recombinant rabbit anti-ERK1/2 [pT185/pY187] monoclonal antibody is used in western blotting, ELISA, and immunohistochemistry.

Antibody Specifications:

• Applications: Validated applications for this rabbit anti-ERK1/2 [pT185/pY187] monoclonal antibody are western blotting, ELISA, and immunohistochemistry.
• Host Species and Isotype: The host species and isotype of the antibody is rabbit IgG.
• Clone ID of Monoclonal Antibody (mAb): The rabbit anti-ERK1/2 [pT185/pY187] monoclonal antibody clone is 15H10L7.
• Reactivity: Reacts with human ERK1/2 [pT185/pY187].
• Product Size: Rabbit anti-ERK1/2 [pT185/pY187] monoclonal antibody is available in a 100 µg size.

ERK (extracellular signal-regulated kinase), also known as MAPK (Mitogen-Activated Protein Kinase), has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli, including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth, differentiation and the cytoskeleton. ERK1 and ERK2 are fully activated by phosphorylation within a Thr-Glu-Tyr motif of the activation loop on both a threonine and a tyrosine residue by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of the ERK1&2 enzymes. Aberrant ERK1/2 phosphorylation has been linked to several cancers and may be a useful prognostic marker in some carcinomas.