Designed for the simple and rapid isolation of high quality PCR-ready genomic DNA from blood. The isolated DNA is free of RNA and contaminants that may inhibit PCR. The isolated DNA is of high integrity and high molecular weight, and PCR-results show excellent reproducibility in combination with a high level of sensitivity. The process of DNA isolation relies upon cell lysis to release the DNA and adsorption of the DNA to the surface of the Dynabeads. This is followed by magnetic separation of the intact DNA/Dynabeads complex and subsequent washing to remove any residual contaminants and potential PCR inhibitors from the isolated DNA. Finally, the complex is resuspended for direct use in downstream PCR-reactions. Alternatively, the DNA may be eluted from the Dynabeads following a short incubation at 65°C. Multiple samples may be handled in parallel and the isolation process can be automated. Benefits and Features:
- Cost-effective, simple and efficient single-tube procedure, completed in only 15 minutes.
- Supports 100 isolations from 100 µl of blood per sample
- Sufficient for at least 100 PCR amplifications
This Product Contains:
- Protocol can be modified to allow isolation from 500 µl blood samples, providing enough template DNA for 1,000 PCR amplifications
Dynabeads® (supplied in Lysis buffer), red cell lysis buffer, washing buffer and resuspension buffer. Applications:
Isolation of PCR-ready genomic DNA directly from blood