The Dynabeads® mRNA DIRECT™ Kit rapidly isolates the mRNA transcriptome directly from any crude sample in 15 minutes. This mRNA isolation kit eliminates the need to purify total RNA when only pure, intact mRNA is the target. Removing this up-front purification step helps save time and minimizes reagent costs associated with total RNA purification.
• Obtain extremely pure mRNA in about 15 minutes
• Integrate total RNA purification and mRNA enrichment steps
• Recover and enrich the transcriptome efficiently
• Prepare mRNA that is suitable for nearly every downstream application

Pure, Rapid mRNA Isolation Directly from Samples
The Dynabeads® mRNA DIRECT™ Kit contains magnetic beads for the isolation of the mRNA transcriptome from a wide variety of crude samples. Isolation of intact mRNA directly from samples, even ones containing RNases, is possible thanks to RNase inhibitiors in the Lysis/Binding buffer, combined with stringent hybridization and washing steps. Ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured resulting in cleaner preparations and more sensitive results. Typically within 15 minutes, pure mRNA can be isolated, ready immediately for use in downstream applications.

A Straightforward Enrichment Procedure
The Dynabeads® mRNA Purification Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads®, coupled to oligo-(dT)₂₅, are first equilibrated with Lysis/Binding Buffer, and then mixed with cell or tissue lysate in order to bind mRNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 10 µl of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes.

Designed for a Broad Range of Sample Types:
Nearly any biological sample of vertebrate, invertebrate, plant, fungal, or bacterial origin can be used with the Dynabeads® mRNA DIRECT™ Kit. The kit is also ideal for extracting mRNA and viral polyadenylated RNA from blood, serum and sputum. Nucleic acid preparations, including total RNA and unfractionated nucleic acid samples, as well as formalin-fixed, paraffin-embedded (FFPE) tissues, may also be extracted using the Dynabeads® mRNA DIRECT™ Kit.

RNA is Suitable for All Downstream Molecular Applications, Including:
• Gene cloning
• cDNA synthesis, cDNA library construction
• RT-PCR, Quantitative RT-PCR
• RPA (Ribonuclease Protection Assay)
• Subtractive Hybridization
• Dot/slot Hybridization
• Primer extension

For Research Use Only. Not for human or animal therapeutic or diagnostic use.