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Interleukin 1 receptor, type I (IL1R1), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse IL1R1 (P13504) (Met 1-Lys 338) fused to a polyhistidine tag at the C-terminus.
Activity: 1. Measured by its ability to bind mouse IL1B in a functional ELISA. 2. Immobilized mouse IL1A at 10 µg/ml (100 µl/well) can bind mouse IL1R1. The EC50 of mouse IL1R1 is 0.13 µg/ml.
Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Expiration Date: Expires one year from date of receipt when stored as instructed.
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