This purified, un-conjugated mouse anti-fibrillarin (Nop1p) monoclonal antibody is specific for human, rat, plant, Drosophila, C. elegans, and S. pombe. This mouse anti-fibrillarin (Nop1p) monoclonal antibody recognizes the expressed product of the FLB gene, also known as FIB, FLRN, RNU3IP1, and FBL. This mouse anti-fibrillarin (Nop1p) monoclonal antibody is used in western blotting and immunofluorescence applications.

Antibody Specifications:

• Applications: Validated applications for mouse anti-fibrillarin (Nop1p) monoclonal antibody are western blotting and immunofluorescence.
• Clone ID of Monoclonal Antibody (mAb): The mouse anti-fibrillarin (Nop1p) monoclonal antibody clone is 38F3.
• Host Species and Isotype: The host species and isotype of the antibody is mouse IgG1.
• Reactivity: Reacts with human, rat, plant, Drosophila, C. elegans, and S. pombe.
• Product Size: Mouse anti-fibrillarin (Nop1p) monoclonal antibody is available in a 100 µl size.

Nop1p was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.