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This purified mouse anti-Phosphoglycerate K monoclonal antibody (mAb) recognizes yeast phosphoglycerate kinase. Phosphoglycerate Kinase (3-Phosphoglyceric Phosphokinase, EC 184.108.40.206) is a soluble protein of the yeast cytoplasm. The Phosphoglycerate Kinase mAb can be used to detect native, denatured, and fixed Phosphoglycerate Kinase. Mouse anti-Phosphoglycerate Kinase mAb is validated for use in applications such as western blotting and immunocytochemistry.
• Applications: Validated applications for mouse anti-Phosphoglycerate Kinase monoclonal antibody are western blotting and immunofluorescence/immunocytochemistry. • Host Species and Isotype: The host species and isotype of the mouse anti-Phosphoglycerate Kinase monoclonal antibody is mouse IgG1, kappa. • Clone ID of Monoclonal Antibody (mAb): The anti-Phosphoglycerate Kinase monoclonal antibody clone is 22C5D8. • Reactivity: Reacts with yeast (S.cerevisiae) • Product Size: Mouse anti Phosphoglycerate Kinase monoclonal antibody is available in a 250 µg pack size.
PGK (3-Phosphoglyceric Phosphokinase, EC 220.127.116.11) is a soluble protein of the yeast cytoplasm. This antibody can be used to detect native, denatured, and fixed PGK. The antibody can be used to visualize the yeast cytoplasm by immunohistochemistry: the nucleus and other organelles exclude staining. Also, since PGK is an abundant fully soluble protein that does not sediment at 200,000 x g, the assessment of the presence or absence of this protein can provide a critical test for cytoplasmic protein contamination of inappropriate subcellular fractions.
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