Anti-ANT (adenine nucleotide transporter) Mouse Monoclonal Antibody is a reversible transporter of ATP (aminophospholipid transporter) and ADP-ribose Polymerase 1. ANT Monoclonal Antibody is specific to human and bovine ANT. Immunoprecipitation is a validated application for use with ANT Monoclonal Antibody.

Antibody Specifications:

• Applications: Immunoprecipitation is the validated application for Anti-ANT Mouse Monoclonal Mitoprofile® Antibody.
• Host Species and Isotype: Mouse IgG1κ is the ANT antibody host species and isotype.
• Clone ID of Monoclonal Antibody (mAb): The monoclonal antibody clone is 5F51BB5AG7.
• Reactivity: Reacts with human and bovine ANT.
• Product Size: Anti-ANT Mouse Monoclonal Mitoprofile® Antibody is supplied in a 250 µg pack size.

ANT is a reversible transporter of ATP and ADP. During oxidative phosphorylation (OXPHOS) the protein exchanges ATP out for ADP in, however, in the absence of OXPHOS, ANT works in reverse to maintain mitochondrial membrane potential. Some patients with heart-related diseases have been shown to harbor mutations in the ANT gene, or to have developed an autoimmune reaction against the protein. There are a number of proteins found in mitochondria that, under circumstances favoring apoptosis, coalesce to form the so-called permeability transition pore (PTP).

The proteins identified as a part of the PTP include cytosolic hexokinase, outer membrane porin (also called the voltage dependent anion channel or VDAC), adenylate cyclase in the intermembrane space, the adenine nucleotide transporter (ANT) and the peripheral benzodiazepine receptor protein of the inner membrane along with cyclophilin-D of the matrix space. Hexokinase, porin and ANT all occur as isoforms and it remains to be determined whether there is specificity of these different forms for the PTP.

Anti-ANT Mouse Monoclonal Antibody is composed of 250 µg of monoclonal antibody irreversibly crosslinked to protein G-agarose beads. Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, then purified by biochemical fractioning.