This rabbit anti-RSK1 [pS221] polyclonal antibody is specific to human, mouse, chicken RSK1 [pS221] and expected to react with rat (not tested). RSK1 [pS221] polyclonal antibody recognizes the expressed product of the RPS6KA1 gene. RSK proteins are substrates for, and downstream transducers of, MAPK signaling proteins, primarily ERK1&2. Validated application for the RSK1 [pS221] polyclonal antibody is Western blotting.
Applications: validated for Western blotting
Host species and isotype: rabbit IgG
Clone ID of monoclonal antibody (mAb): 998A2A2
Reactivity: human, chicken, mouse, (rat) RSK1 [pS221]
Product size: 10 blots pack size
RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2
(MAPKAP-K1b), RSK3 (MAPKAP-K1c) and RSK4. RSK proteins are substrates for, and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of complex formation with ERK1&2 and/or 14-3-3β proteins. RSK proteins are activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK1&2 phosphorylate threonine 573 (threonine 577 in RSK2) of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 (serine 386 in RSK2) within the linker region. This is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 (serine 227 in RSK2) and thereby activates the Nterminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators.