This purified Rabbit anti-c-Met [pYpYpY1230/1234/1235] Polyclonal Antibody recognizes c-Met that is phosphorylated at tyrosine residues 1230, 1234 and 1235. Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c-Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c-Met contains three tyrosines (Tyr-x-x-x-Tyr-Tyr motif) within the activation loop of the catalytic domain. The Rabbit anti-c-Met [pYpYpY1230/1234/1235] Polyclonal Antibody is validated for use in applications such as Western Blot, Immunohistochemistry, and Immunocytochemistry/Immunofluorescence.

Antibody Specifications:

• Applications: Validated applications for Rabbit anti-c-Met [pYpYpY1230/1234/1235] Polyclonal Antibody are Western Blotting, Immunohistochemistry, and Immunocytochemistry/Immunofluorescence.
• Host Species and Isotype: The host species and Isotype of the antibody is rabbit IgG.
• Reactivity: Anti-cMet Polyclonal Antibody reacts with human, mouse and rat (predicted) c-Met phosphorylated at tyrosine residues 1230, 1234, and 1235.
• Product Size: Rabbit anti-c-Met [pYpYpY1230/1234/1235] Polyclonal Antibody is available as a 10 mini-blot pack size.

Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c-Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core, and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity, and multisubstrate docking. c-Met contains three tyrosines (Tyr-x-x-x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c-Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c-Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-γ, and STAT3).