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The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers 2) Library capture onto Equalizer™ Beads 3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow • Seamlessly integrates into your existing library preparation workflow • Reduced pipetting compared to the Agilent 2100 Bioanalyzer®-based workflow • Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries • Minimizes library-to-library variability
Cost Effective • No additional capital equipment required • Less expensive than Agilent 2100 Bioanalyzer®-based workflow (up to 10 samples) • Reduced AMPure® XP reagent usage compared to Agilent 2100 Bioanalyzer®-based workflow • Long shelf life: up to 18 months • Convenient 96-reaction kit helps reduce ordering frequency
Flexible • Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors • Use with a single sample or in a 96-well plate format • Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes: The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow: Ion AmpliSeq™ Library Kit 2.0 Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com) --OR-- Ion Plus Fragment Library Kit --OR-- Ion Xpress™ Plus Fragment Library Kit
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