This purified, unconjugated EGFR [pY1173] Rabbit polyclonal antibody is specific to human EGFR [pY1173], with expected reaction to mouse, rat, and chicken. Epidermal Growth Factor Receptor, also known as ErbB1 (EGFR, a 185 kDa glycoprotein) is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. Mouse anti-EGFR [pY1173] polyclonal antibody is used in western blotting, ELISA, and immunocytochemistry applications.
• Applications: Validated application for Mouse anti-EGFR [pY1173] polyclonal antibody is western blotting.
• Host Species and Isotype: The host species and isotype of the antibody is rabbit IgG.
• Aliases: EGFR is also known as ERBB, ERBB1, HER1, PIG61, and mENA.
• Reactivity: Reacts with human EGFR [pY1173].
• Product Size: Rabbit anti-EGFR [pY1173] polyclonal antibody is available in a 10 mini blot pack.
Epidermal Growth Factor Receptor, also known as ErbB-1 (EGFR, a 185 kDa glycoprotein) is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. The EGFR consists of a large extracellular domain, a single trans-membrane domain and a cytoplasmic domain that exhibits kinase activity. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. Autophosphorylation of tyrosine 1173 creates a major binding site for the protein tyrosine phosphatase SHP-1, which can dephosphorylate the EGFR and thereby block EGFR-induced activation of the ERK1&2 signaling pathway.
Mouse anti-EGFR [pY1173] was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated EGFR. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1173.