Ion TargetSeq™ Exome kits provide a solution-phase DNA probe capture technology for targeted resequencing applications that enables selective, specific, and economical enrichment of exons and other important regions within the human genome. The capture protocol and capture probe content have been thoroughly optimized to give superior performance on both Ion Proton™ and PGM™ sequencing instruments. In a single tube the kits enable multiplexed sample capture, which provides an efficient and economical solution for sequencing and variant detection in all types of disease-related samples.Highly optimized probe design algorithms and industry-leading probe densities result in more desirable target coverage metrics:
• Improved evenness in coverage as measured by percent of reads covered at 1x, 10x, and 20x depths
• Greater percentage of “on target" reads
• Higher number of SNPs detected at 10x and 20x coverage depths as compared to competitive products
• Higher percent of SNP concordance with dbSNP at 10x and 20x coverage depths as compared to competitive products
Integrated and Cost-effective Capture Solution
Ion TargetSeq™ enrichment products, like the Ion TargetSeq™ Exome kits, are optimized to provide exceptional and economic performance when combined with Ion Xpress™ Plus library kits, the AB Library Builder™ automated library construction instrument, and Ion Proton™ sequencing system. Ion TargetSeq™ Exome kits are some of the first targeted resequencing products to allow multiplex sample capture using library barcoding and pre-capture sample pooling, which dramatically lower the per sample costs and simplify the workflow. The Ion TargetSeq™ Exome kits are part of an end-to-end solution for targeted resequencing that is automation friendly and contains simplified data analysis tools with the Torrent Suite software and the variant calling plug-in.
Low Sample Input Required for Efficient Target Enrichment
Ion TargetSeq™ Exome kits employ a capture protocol that requires as little as 500 ng of individual or pooled barcoded libraries, helping to conserve precious clinical samples. The Ion TargetSeq™ protocol allows users to leverage barcoded fragment libraries to provide fast, efficient, and economical sample enrichment prior to sequencing.
Comprehensive Capture Probe Content
The genomic regions that encompass the capture probe content were identified using the latest versions of human gene expression, genome annotation, and cancer databases including RefSeq, CCDS, VEGA, GenCode, Ensembl®, and COSMIC. Specifically, the targeted regions consist of 39.5 Mb of exons, 1472 miRNA genes, >44,000 miRNA binding sites in 3’-UTRs, various tRNAs, snoRNAs, lncRNAs, and >97% of the genes variants in the COSMIC database.
Everything Needed for Target Enrichment
Ion TargetSeq™ Exome kits are conveniently packaged with all the reagents needed to perform target enrichment, including all hybridization and wash buffers, Human Cot-1 DNA® Fluorometric QC, Dynabeads® M-270 Streptavidin magnetic beads, and blocker oligos for standard fragment library adapters, paired-end library adapters, and Ion Xpress™ barcoded library adapters (BCs 1-16). These enrichment kits provide highly specific and reproducible enrichment of 4 to 192 samples with unparalleled coverage metrics (e.g., >90% uniformity of coverage, >75% of reads on target with bases covered at 20x depth).