The TaqMan® OpenArray® Human Signal Transduction Panel was developed to identify differentially expressed genes involved in major signaling pathways. This gene signature panel contains 573 TaqMan® assays specific to signal transduction-related genes plus 24 endogenous controls. The format of the OpenArray® plate allows for 1–4 replicates to be run in parallel per plate.
In this real-time PCR assay panel, genes encoding the JAK-STAT, NFκB, Akt, GPCR, cAMP, and MAP kinase pathways are well represented. The full lengths of these pathways are covered, from ligand to receptor to kinase to transcription factor. In addition, there are 18 endogenous control genes against which the assays can be normalized. All assays in this panel have been tested in our laboratories, so you can be sure that each assay is fully optimized.
The plate is pre-formatted and inventoried, cost-effective, convenient, and easy-to-use, without the need for expensive robotics. Results are reproducible and consistent across samples, studies, and labs—for the same data quality from card-to-card and lot-to-lot, even with different operators.
The Right Targets
This OpenArray® plate uses the proprietary Applied Biosystems® OpenArray® technology to simultaneously analyze gene expression levels by quantitative PCR for 584 test genes and 20 endogenous controls.
Genes were included based on the following criteria:
• Expression in human embryonal carcinoma cells (hEC), primordial germ cells, or human ES (hES) cells
• Known role in maintenance of pluripotency in hES cells
• Identification in microplate-based experiments as being correlated with the cancer state
• Expression known to be characteristic of specific differentiation lineages in the post implantation embryo and mature tissues
This panel is intended for use with the QuantStudio™ 12K Flex Real-time PCR system, which offers high throughput profiling of 5,184 samples per work day, generating 43,000 expression profiles without expensive robotics while saving precious sample and lowering reagent costs.