The TaqMan® OpenArray® Human Stem Cell Panel is an efficient, easy-to-use OpenArray® plate for the characterization of undifferentiated stem cells or their differentiated derivatives. This gene signature panel targets 609 well-defined genes validated as markers for pluripotency, plus 22 endogenous control genes. The format of the OpenArray® plate allows for 1–4 replicates to be run in parallel per plate.
The TaqMan® OpenArray® Human Stem Cell Panel contains a well-defined set of validated gene expression markers to characterize human embryonic stem (ES) cell identity and assess phenotypic variations between embryonic stem cell isolates. The gene content of this OpenArray® plate resulted directly from the work of the I.S.C.I consortium and characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
The plate is pre-formatted and inventoried, cost-effective, convenient, and easy-to-use, without the need for expensive robotics. Results are reproducible and consistent across samples, studies, and labs—for the same data quality from card-to-card and lot-to-lot, even with different operators.
The Right Targets This OpenArray® plate uses the proprietary Applied Biosystems® OpenArray® technology to simultaneously analyze gene expression levels by quantitative PCR for 612 test genes and 22 endogenous controls.
Genes were included based on the following criteria:
• Expression in human embryonal carcinoma cells (hEC), primordial germ cells, or human ES (hES) cells • Known role in maintenance of pluripotency in hES cells • Identification in microplate-based experiments as being correlated with the stem cell state • Expression known to be characteristic of specific differentiation lineages in the post implantation embryo and mature tissues
This panel is intended for use with the QuantStudio™ 12K Flex Real-time PCR system, which offers high throughput profiling of 5,184 samples per work day, generating 43,000 expression profiles without expensive robotics while saving precious sample and lowering reagent costs.
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