The Attune® Acoustic Focusing Cytometer Auto Sampler Upgrade Kit is an optional accessory for upgrading existing Attune® Acoustic Focusing Cytometers for use with the new Attune® Acoustic Flow Cytometer Auto Sampler. This upgrade kit is intended for cytometer models that were not originally designed for use with this device (catalog numbers 4445315 and 4469120) This upgrade kit includes the auto sampler, a computer, and the Attune® Auto Sampler Accessory Kit. The Attune® auto sampler enables rapid processing of multiple samples by automatically analyzing samples from 96- or 384-well standard and deep well plates.
Some features of the Attune® auto sampler are:
• Flexible—accomodates multiple plate formats
• Reliable—instrument design results in higher productivity and less down time
• Accurate—sample mixing leads to consistent results
• Simple to use—experiment setup has never been this simple
The Attune® auto sampler allows use of many different plate formats, including 96- and 384-well standard and deep-well plates, without being restricted to a single manufacturer. To maintain system flexibility, the manual sample probe is always accessible even when the loader is present, and switching between tubes and plates is as simple as a flip of a switch.
The Attune® auto sampler was designed to minimize system down time. Automated sampling devices are often plagued by clogging or probe crashes. The Intelliprobe™, with a 200 micron diameter that is larger than most probes available in the industry, minimizes instances of clogging.
To prevent probe crashes, which may require expensive probe replacement, the Attune® auto sampler automatically performs a probe self-calibration every month to ensure consistent positioning of the probe within every well. The self-calibration verifies proper function of the probe, reduces variability in probe positioning over time to prevent failures during plate runs, and improves reliability over the life of the system. Self-calibration of the probe also allows the auto sampler to be easily moved, so it can be shared between multiple instruments.
In addition, the Attune® auto sampler initiates automated cleaning procedures when the instrument is shutting down to ensure proper maintenance with little user interaction.
Unlike other plate loaders that mix samples by shaking, which may not ensure homogeneity of the sample, the Attune® auto sampler mixes samples by aspiration, ensuring uniform mixing while preserving sample viability and resulting in consistent data throughout the plate.
Simple to Use
The instrument design and software were developed with customer ease-of-use in mind. The Attune® auto sampler has fluidic bottles with sensors that inform the user when the fluids need to be replaced. The auto sampler’s indicator lights and warning systems have been designed to ensure that a user who has walked away from the system can easily see the system status at all times.
Setting up an experiment with the Attune® cytometric software is simple. Using a virtual plate layout view, users can create compensation wells, define instrument settings, and identify samples. The software also allows multiple experiments to be defined on a single plate. The intuitive software allows quick learning and data analysis. For example, a heat map view allows rapid screening and confirmation of samples while the instrument is acquiring data.
The software is free and downloadable online at www.lifetechnologies.com/attune. Multiple users can access the software. No license fees or dongles are required.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.