Poros® CaptureSelect® LC Kappa HPLC columns incorporate the advantages of high-speed Poros® chromatography resins with the unique selectivity of CaptureSelect® ligands. The combination allows three minute quantitation of Fabs, immunoglobulins, and bispecific antibodies having a Kappa light chain.
• Broad utility—POROS® A, G and CaptureSelect®affinity ligands address most biological drug classes
• Rapid—POROS® resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—deliver high-performance purification over hundreds of chromatography cycles
High Speed with High Specificity
The combination of CaptureSelect® LC Kappa affinity ligands when combined with POROS® 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of all immunoglobulins and Fabs having a Kappa light chain (all subclasses).
POROS® perfusion chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry. Figure 2 illustrates the chromatographic performance of POROS® CaptureSelect® Kappa with five unique kappa light chain molecules. The associated standard curves, Figure 3, exhibit excellent linearity.
POROS® affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS® Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
LC Kappa Column Specifications:
|Protein Binding Capacity||14.5 mg/mL|
|Column Material||PEEK™ polymer or stainless steel|
|Frit||2 µm, titanium|
|POROS® Resin||Cross-linked poly (styrenedivinylbenzene)|
|CaptureSelect® Ligands||Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae|
For Research Use Only. Not for use in diagnostics procedures.