- Remove xylene and deparaffinization from your FFPE DNA purifications
- Purify DNA from FFPE tissue samples in a high-throughput platform using the kit’s magnetic bead-based technology
- Get DNA in 3 hours (automated) or 3.5 hours (manual)
- Achieve superior performance with our simplified workflow in one complete kit
Existing methods utilize xylene or similar solvents followed by alcohol washes to solubilize the paraffin before undergoing a protease digestion and then clean-up with glass fiber filter spin columns. While effective, these methods employ toxic chemicals and require considerable hands-on time. The MagMAX™ FFPE DNA Isolation Kit employs novel chemistries to remove the need for these hazardous solvents by removing the deparaffinization step entirely. A streamlined protocol consisting of solution additions (as opposed to transfers or other steps) consolidates and makes the process simpler, faster, and safer.
Scale and Throughput - Finally
The MagMAX™ FFPE DNA Isolation Kit utilizes magnetic bead chemistry which enables users to scale up to 96 samples at a time. Manual extractions are best performed in a 96-well plate with commonly available magnetic stands and multichannel pipets in approximately 3.5 hours, with approximately 1.5 hours hands-on time. For automated processing, the 3 hour protocol requires only 30 minutes of hands-on time to perform the improved protease-digestion and bead-binding steps, before the sample is placed on the MagMAX™ Express-96 Magnetic Particle Processor for the remaining wash, on-bead nuclease treatment, and elution steps. The process can also be automated on open-format robotics.
Efficient DNA Recovery Without Overnight Digestion
The MagMAX™ FFPE DNA Isolation Kit purifies DNA from up to two 10 µm sections per well with yield comparable to the best-in-class Ambion RecoverAll™ kit and better than competitive offerings. Whereas typical DNA purification requires an overnight digestion step, the MagMAX™ FFPE DNA Isolation Kit requires only a 1.5 hour digestion, significantly reducing the total processing time.
Purified DNA Ready for Downstream Analysis
Recovered DNA is suitable for downstream applications such as PCR, microarray analysis, and mutation screening. However, as is the case with all FFPE tissue, sample fixation and storage typically cause nucleic acid fragmentation and modification. Therefore, downstream applications may require modification for best results.
Complete Kit Convenience
The MagMAX™ FFPE DNA Isolation Kit contains everything needed to perform 96 purifications. RNase, proteinase, magnetic beads, and all wash and elution buffers are supplied. A 96-well processing plate and a 96-well elution plate are also included for convenience.
Need both RNA and DNA from Your FFPE Samples?
A stand-alone MagMAX™ Total Nucleic Acid Isolation Kit is available separately for users who are working with both RNA and DNA.