- Remove xylene and deparaffinization from your FFPE nucleic acid purifications
- Purify total nucleic acids, including miRNA, from FFPE tissue samples in a high-throughput platform using the kit’s magnetic bead-based technology
- Get RNA or DNA in 3 hours (automated) or 3.5 hours (manual)
- Achieve superior performance with our simplified workflow in one complete kit
Existing methods utilize xylene or similar solvents followed by alcohol washes to solubilize the paraffin before undergoing a protease digestion and then clean-up with glass fiber filter spin columns. While effective, these methods employ toxic chemicals and require considerable hands-on time. The MagMAX™ FFPE Total Nucleic Acid Isolation Kit employs novel chemistries to remove the need for these hazardous solvents by removing the deparaffinization step entirely. A streamlined protocol consisting of solution additions (as opposed to transfers or other steps) consolidates and makes the process simpler, faster, and safer.
Scale and Throughput—Finally
The MagMAX™ FFPE Total Nucleic Acid Isolation Kit utilizes magnetic bead chemistry which enables users to scale up to 96 samples at a time. Manual extractions are best performed in a 96-well plate with commonly available magnetic stands and multichannel pipets, in approximately 3.5 hours, with approximately 1.5 hours of hands-on time. For automated processing, the 3 hour protocol requires only 30 minutes of hands-on time to perform the improved protease-digestion and bead-binding steps, before the sample is placed on the MagMAX™ Express-96 Magnetic Particle Processor for the remaining wash, on-bead nuclease treatment, and elution steps. The process can also be automated on open-format robotics.
Total Nucleic Acid Recovery
The MagMAX™ FFPE Total Nucleic Acid Isolation Kit can purify both total RNA (including miRNA) as well as DNA from the same kit. Up to two 10 µm sections can be processed per well with yield comparable to the best-in-class Ambion® RecoverAll™ Kit and better than competitive offerings. miRNA, which is least affected by the cross-linking process, is effectively co-purified with the mRNA, further enabling the utility of this otherwise challenging sample. Whereas typical DNA purification requires an overnight digestion step, the MagMAX™ FFPE Total Nucleic Acid Isolation Kit requires only a 1.5 hour digestion, significantly reducing the total processing time.
Purified Nucleic Acid Ready for Downstream Analysis
Recovered nucleic acids are suitable for downstream applications such as qRT-PCR, microarray analysis, and mutation screening. However, as is the case with all FFPE tissue, sample fixation and storage typically cause nucleic acid fragmentation and modification. Therefore, downstream applications such as microarray analysis, which require more pristine RNA than does qRT-PCR, may require modification for best results.
Complete Kit Convenience
The MagMAX™ FFPE Total Nucleic Acid Isolation Kit contains everything needed to perform 96 purifications. DNase and RNase are both supplied for maximum user flexibility and are employed in an efficient on-bead nuclease treatment. A 96-well processing plate and a 96-well elution plate are also included for convenience.
Need Only DNA from Your FFPE Samples?
A stand-alone MagMAX™ FFPE DNA Isolation Kit is available separately for users who are working solely with DNA and are not interested in RNA purification.