AKT⁄PKB [pS473], Rabbit Monoclonal Antibody - UNCONJ

Cat. No. 44-621G

For improved reproducibility and sensitivity please try AKT [pS473] ABfinity™ Recombinant Rabbit Monoclonal Antibody Catalog# 700392.
AKT⁄PKB [pS473] MAb human, mouse (Invitrogen); Unconjugated Monoclonal antibody specific to Human, More

Manuals
MSDS

General Specifications

Host:	Rabbit
Label or Dye:	Unconjugated
Product Size:	10 blots
Reactivity:	Mouse Human
Target Gene:	Akt/PKB [pS473]
Target Modification State:	Phospho
Validated Application:	Immunohistochemistry Western Blotting

Top Material Safety Data Sheet (1)

44621G

Image Gallery (2)

Image Gallery (2)

Immunofluorescence Staining Serum-starved NIH3T3 cells left untreated (left) or treated with PDGF (right) were fixed prior to immunostaining with the Akt [pS473] rabbit monoclonal antibody.

Validation of LanthaScreen™ and CellSensor® cell lines via western blot.

Validation of LanthaScreen™ and CellSensor® cell lines via western blot. (A) LanthaScreen™ GFP-AKT HEK 293E cells were treated under the indicated conditions, including IGF-1 (Cat. no. PHG9071), LY294002 (Cat. no. PHZ1144), and wortmannin (Cat. no. PHZ1301). Cell extracts were resolved by SDS-PAGE and transferred to nitrocellulose with the iBlot® Gel Transfer Device (Cat. no. IB1001). The membrane was then probed with the anti-AKT [PS473] PSSA (Cat. no. 44-621G), and the signal was detected using a WesternBreeze® Chromogenic Kit (Cat. no. WB7105). (B) CellSensor® T REx™ FOXO3 DBE-bla HeLa cell lysates were generated by treatment with doxycycline to induce FOXO3 expression, followed by incubation with the indicated stimulants or inhibitors and lysis. The nitrocellulose membrane was prepared and developed as in (A), except that the PSSA used in this case was anti-FOXO3 [PT32] (Cat. no. 44-1240G).

Related Applications

Product Systems

Target Modified Antibodies

AKT⁄PKB [pS473], Rabbit Monoclonal Antibody - UNCONJ

Manuals (1)

Material Safety Data Sheet (1)

Manuals (1)

General Specifications

Rabbit

Unconjugated

10 blots

Mouse

Human

Akt/PKB [pS473]

Phospho

Immunohistochemistry

Western Blotting

Top Material Safety Data Sheet (1)

Image Gallery (2)

Immunofluorescence Staining Serum-starved NIH3T3 cells left untreated (left) or treated with PDGF (right) were fixed prior to immunostaining with the Akt [pS473] rabbit monoclonal antibody.

Validation of LanthaScreen™ and CellSensor® cell lines via western blot.