The Attune™ Shutdown Solution is a ten-fold concentrated solution that is diluted 1:10 in sterile deionized water and used in the automated shutdown procedure for the Attune™ Acoustic Focusing Cytometer. This azide-free shutdown solution has been formulated to minimize bubble formulation that can occur in the system lines overnight, to help keep the fluid lines clean, and to minimize microbial growth in the fluid lines.
The Shutdown cycle is used at the end of each day to ensure that all sample fluids and dyes have been removed from the system, and that the fluidic lines and pumps are filled with Attune™ Shutdown solution to prevent salt deposits from forming in the fluidics lines. Shutdown takes around 20 minutes, but most of the steps are automatic under the control of the Attune™ Cytometric Software. Shutdown procedures should be run at least once a day, even if the instrument is intended for continuous use. Proper cleaning of the instrument ensures its consistent and accurate operation.
For more information about the Attune™ Acoustic Focusing Cytometer, please go here