c-KIT [pY721], Rabbit Polyclonal Antibody - UNCONJ

Cat. No. 44-494G

c-KIT [pY721] PAb; Unconjugated Polyclonal antibody specific to Human (Mouse, Rat) c-Kit [pY721]. This antibody is validated for use in Western Blot. Anti-c-Kit [pY721] recognizes the expressed product of the KIT gene.

Catalog number 44494G is same antibody as former catalog number 44494. 44494G contains 50% glycerol, which improves the stability and longevity of this antibody.

Manuals
MSDS

General Specifications

Host:	Rabbit
Label or Dye:	Unconjugated
Product Size:	10 blots
Reactivity:	Human
Target Gene:	c-Kit [pY721]
Target Modification State:	Phospho
Validated Application:	Western Blotting

Top Material Safety Data Sheet (1)

44494G

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Image Gallery (1)

Up-regulation and Antibody-Peptide Competition.

Peptide Competition and Stimulation. Extracts prepared from Mo7e cells left untreated (1) or treated with 100 ng/mL human SCF for 5 minutes (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with the c-Kit [pY721] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase (Cat. no. ALI4405) and signals were detected using the Tropix WesternStar™ method. The data show that only the phosphopeptide corresponding to c-Kit [pY721] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of c-Kit [pY721] phosphorylation by the addition of SCF to this cell system.

Related Applications

Product Systems

Target Modified Antibodies

c-KIT [pY721], Rabbit Polyclonal Antibody - UNCONJ

Manuals (1)

Material Safety Data Sheet (1)

Manuals (1)

General Specifications

Rabbit

Unconjugated

10 blots

Human

c-Kit [pY721]

Phospho

Western Blotting

Top Material Safety Data Sheet (1)

Image Gallery (1)

Up-regulation and Antibody-Peptide Competition.