C-FOS [pT232], Rabbit Polyclonal Antibody - UNCONJ

Cat. No. 44-280G

C-FOS [pT232] PAb; Unconjugated Polyclonal antibody specific to Human, (Mouse, Rat) c-Fos [pT232]. This antibody is validated for use in Western Blot. Anti-c-Fos [pT232] recognizes the expressed product of the FOS gene.

Catalog number 44280G is same antibody as former catalog number 44280. 44280G contains 50% glycerol, which improves the stability and longevity of this antibody.

Manuals
MSDS

General Specifications

Gene ID:	2353
Host:	Rabbit
Label or Dye:	Unconjugated
Product Size:	10 blots
Reactivity:	Human
Target Gene:	c-Fos [pT232]
Target Modification State:	Phospho
Validated Application:	Western Blotting

Top Material Safety Data Sheet (1)

44280G

Image Gallery (1)

Image Gallery (1)

Up-regulation and Antibody-Peptide Competition.

Peptide Competition. Extracts of SKBR3 cells were treated with 200ng/mL EGF for 10 minutes and resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 3% Milk-TBST buffer for one hour at room temperature, then incubated with the c-Fos [pT232] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate (Cat. no. ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to c-Fos [pT232] blocks the signal, demonstrating the specificity of the antibody. While c-Fos [pT232] is phosphorylated in the basal state of SKBR3 (data not shown), EGF treatment induced an increase in the phosphorylation signal.

Related Applications

Product Systems

Target Modified Antibodies

C-FOS [pT232], Rabbit Polyclonal Antibody - UNCONJ

Manuals (1)

Material Safety Data Sheet (1)

Manuals (1)

General Specifications

2353

Rabbit

Unconjugated

10 blots

Human

c-Fos [pT232]

Phospho

Western Blotting

Top Material Safety Data Sheet (1)

Image Gallery (1)

Up-regulation and Antibody-Peptide Competition.