AMPK-α; 1⁄2 [pT172], Rabbit Polyclonal Antibody - UNCONJ

Cat. No. 44-1150G

AMPK-ALPHA 1⁄2 [pT172] PAb; Unconjugated Polyclonal antibody specific to Human, Mouse, Rat AMPK-α 1&2 [pT172]. This antibody is validated for use in Western Blot. Anti-AMPK-α 1&2 [pT172] recognizes the expressed product of the PRKAA1 gene.

Catalog number 441150G is same antibody as former catalog number 441150. 441150G contains 50% glycerol, which improves the stability and longevity of this antibody.

Manuals
MSDS

General Specifications

Host:	Rabbit
Label or Dye:	Unconjugated
Product Size:	10 blots
Reactivity:	Mouse Human
Target Gene:	AMPK-alpha 1/2 [pT172]
Target Modification State:	Phospho
Validated Application:	Western Blotting

Top Material Safety Data Sheet (1)

441150G

Image Gallery (1)

Image Gallery (1)

Up-regulation and Antibody-Peptide Competition.

Upregulation, Antibody-Peptide Competition and Phosphatase Stripping. Extracts of HepG2 cells untreated (1) or treated with 12 mM Metformin for 24 hours in serum free media (2-6) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 3% BSA-TBST buffer for one hour at room temperature, and then incubated with the AMPKalpha 1/2 [pT 172] antibody for two hours at room temperature in 3% BSA-TBST buffer, following prior incubation with: no peptide (1,2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate (Cat. no. ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to AMPKalpha 1/2 [pT172] completely blocks the signal and that phosphatase stripping eliminates the signal, verifying that the antibody is phosphorylation site-specific. The data also show upregulation of the phospho-signal upon Metformin treatment in this cell system.

Related Applications

Product Systems

Target Modified Antibodies

AMPK-α; 1⁄2 [pT172], Rabbit Polyclonal Antibody - UNCONJ

Manuals (1)

Material Safety Data Sheet (1)

Manuals (1)

General Specifications

Rabbit

Unconjugated

10 blots

Mouse

Human

AMPK-alpha 1/2 [pT172]

Phospho

Western Blotting

Top Material Safety Data Sheet (1)

Image Gallery (1)

Up-regulation and Antibody-Peptide Competition.