- Simplest method available to study copy number variation
- Over 1.6 million pre-designed assays available to cover gene targets, known copy number variations, and extragenic/non-gene regions
- Measure distinct copy number changes quantitatively with high specificity and reproducibility
Custom-designed assays can be generated using Applied Biosystems®´ proven algorithms for genomic targets not covered by the pre-designed assay collection. Please see the Applied Biosystems® website for details on the Custom TaqMan® Copy Number Assays.
TaqMan® Copy Number Assays consist of a TaqMan® minor groove binding (MGB) probe labeled with FAM™ dye and unlabeled PCR primers. TaqMan® Copy Number Assays are run simultaneously with a choice of TaqMan® Copy Number Reference Assays (RNase P or TERT using VIC® dye-labeled TAMRA™ probes) in a duplex real-time polymerase chain reaction. The copy number assay detects the target gene or genomic sequence of interest, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. Relative quantitation analysis is performed by CopyCaller™ Software, using either a known calibrator sample or no calibrator sample method.
Simplest Workflow Available
TaqMan® Copy Number Assays have the simplest workflow of all currently available CNV analysis methods. The test assay (FAM™ dye-labeled), the reference assay (VIC® dye-labeled), your sample DNA, and TaqMan® Master Mix are combined and then run on an Applied Biosystems® real-time PCR system using the standard TaqMan® Genotyping Assay protocol. On average, setup to primary analysis takes only 3–4 hr (including an approximately 2 hr PCR run).
See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.