The Applied Biosystems® AmpliTaq Gold® 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package, with all components premixed and premeasured. AmpliTaq Gold 360 Master Mix was designed for 360° coverage of a full range of targets. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers. Designed for convenience, the AmpliTaq Gold 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase but it also contains the world-class 360 GC Enhancer which can be added optionally for high GC content templates. With the wide template coverage, optimization of PCR reaction conditions, is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity, specificity, and yield
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data
* Easy-to-use, pre-mixed master mix
Optimized for Easy and Challenging Targets
Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).
Description AmpliTaq Gold® 360 Master Mix Specific Yield (ng) AmpliTaq Gold® 360 Master Mix Specificity (%) QIAGEN HotStar® Taq Master Mix Specific Yield (ng) QIAGEN HotStar® Taq Master Mix Specificity (%)
Average without GC-rich Amplicons 2,979.53 97.11 2,856.09 96.3
Average across GC-rich Amplicons 3247.11 51.36 152.96 64.20
Average across all Amplicons 3064.67 69.14 1,996.00 86.43
As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.
Optimized for Automated, Hot-Start PCR
AmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated, convenient and efficient hot-start PCR. When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured.
The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1. The exquisite specificity allows easier multiplexing and allelic discrimination.
Amplify Low Copy Amplicons and Long Targets
AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 2), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 3 demonstrates robust PCR amplification of long human and plasmid DNA.
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.