The Applied Biosystems® AmpliTaq® 360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq® DNA polymerase, AmpliTaq® 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates including those from bacterial and human genomes.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity and yield
* Market-leading GC enhancer for robust amplification of GC-rich sequences
* Achieves the highest quality sequencing data
AmpliTaq® 360 DNA Polymerase has been extensively tested and optimized across a broad panel of difficult targets for best-in-class performance. Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be amplified reproducibly with a single reagent under standardized conditions.
Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Figure 1). As shown in Figure 1, GC-rich regions are poorly amplified with other DNA polymerases while AmpliTaq 360 DNA Polymerase provides successful, robust amplification. These data are shown in a graphical format for specificity (Figure 2a) and specific yield (Figure 2b). The data averaged over all the targets is summarized in Table 1.
Superior Length and Sensitivity
AmpliTaq 360 DNA Polymerase reproducibly and efficiently amplifies long (up to 5 Kb) sequences. Figure 3 demonstrates high quality PCR amplification of long human and plasmid DNA. AmpliTaq 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 4), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, multiplex PCR, allelic discrimination and amplification of targets from degraded DNA samples. The extreme purity of the enzyme, along with its validated and optimized buffer system, contributes to its unmatched sensitivity.
Superior Sensitivity, Reproducibility and Yield
Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Buffer, this ultra-pure enzyme, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes. This provides excellent specificity, reproducibility and yield across a broad range of targets (Figure 1).
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.