Copy and paste this HTML to embed a link in your website
Paste link in email or IM
This product is High Resolution Melting (HRM) Software version 2.0. The HRM Software is now available on the Applied Biosystems 7500 Fast System and the 7900HT Fast Real-Time PCR System. The 7500 Fast Real-Time PCR System delivers precise results with fast thermal cycling in a standard 96-well format. Achieve high-throughput HRM analysis with the 384-well 7900HT, the gold standard high throughput system.
High Resolution Melting (HRM) analysis is an alternative to dHPLC sequencing screening of new gene variants. The AB HRM application does not require temperature shifting, which results in a greater likelihood of identifying new homozygous mutations than methods that require temperature shifting.
• Shorten analysis time by auto-calling genotypes and automatically omitting the no template controls • Minimize subject analysis by automatically grouping unknown variant clusters • Enable easy data review with customizable plot views, expandable windows, and one-click color assignment to highlight curves of interest. • Ability to analyze multiple targets (assays) on one plate Identify more new variants, quickly and accurately The ability to easily identify new variants is key for successful HRM applications. By eliminating the temperature shift step, the Applied Biosystems HRM solution (Fig 1) was able to clearly distinguish homozygous variant samples from homozygous wildtype samples in 97.5% of the population, whereas the other HRM system from Competitor R (Fig 2) was only able to distinguish them in 10% of the population. All genotypes were auto-called by the respective software packages and were not altered by the operator. Class 1 SNP (A/G), multiple technical replicates of nine DNA samples representing three genotypes: homozygous wildtype (G/G), homozygous mutant (A/A) and heterozygous (A/G).
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.