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Applied Biosystems®TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.
TaqMan® PreAmp Master Mix lets you:
• Analyze mRNA from any precious sample such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression Assays • Analyze up to 100 gene expression targets with minimal hands-on time
Preserves Equilibrium of Targets Without Preamplification Bias In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.
Easy Workflow With TaqMan® PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan® Assays , preamplification setup (mixing the pooled assays with the cDNA sample and the TaqMan® PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a 9700 thermal cycler.
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