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The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes.
Features of the High Capacity RNA-to-cDNA™ Kit:
• Fast—Short reaction time (typically 30–60 min) • Convenient—Easy workflow with few pipetting steps (2 tubes) • Reliable—Reliable reverse transcription of both abundant and limited targets • Flexible—Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction
Streamline Your cDNA Synthesis while Maintaining Detection Sensitivity The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template input amounts. The amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than does the previous High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.
For Research Use Only. Not for use in diagnostic procedures.
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