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The Ambion® TaqMan® Cells-to-CT Control Kit is designed for use with the TaqMan® Gene Expression Cells-to-CT Kit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan® Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan® Gene Expression Assay for the highly expressed endogenous control gene β-actin.
Features of the The Ambion® TaqMan® Cells-to-CT Control Kit: • Confirms sufficient cell input • Monitors the presence of RT or real-time PCR inhibitors • Controls for sample normalization
Monitor Inhibition and Confirm Sufficient Cell Input The TaqMan® Cells-to-CT™ Control Kit is used to assess inhibition and cell input in Cells-to-CT reactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan® Gene Expression Assay.
Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ~100 cells and monitors the effectiveness of cell lysis.
Evaluate Compatibility with New Cell Lines The TaqMan® Cells-to-CT™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan® Gene Expression Cells-to-CT Kit to determine the maximum number of cells that can be used per lysis reaction.
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