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Fast SYBR® Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency. • Fast—Real-time PCR results in as fast as 35 minutes • Sensitive—Detect very low copies of target • Specific—Minimize primer-dimer and non-specific amplification • Reproducible—Consistent amplification across a wide dynamic range Fast SYBR® Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer: • AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature • SYBR® Green I dye to enable detection of double-stranded DNA • Deoxynucleotides (dNTPs) to help maintain optimal PCR results • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination • Passive internal reference based on proprietary ROX™ dye to enable increased precision
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