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The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 200 x 20 µL reactions.
• Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits
cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg input total RNA. Ideal for generating cDNA archives. Downstream applications include real-time PCR, standard PCR, and microarrays.
Extensively Tested with a Variety of Templates Quantitative first strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
Accessory Products Available separately, and at no cost are the High Capacity cDNA Reverse Transcription Kit Protocol (Catalog #4375575).
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