This mouse anti-NF-kappa-B (p65) monoclonal antibody is specific to human NF-kappa-B (p65) protein and expected to react with monkey, chimpanzee, rat, dog, cow, horse, and mouse (not tested). Nuclear factor-κBs are transcription factors that consist of homo- and heterodimers of NFκB1/p50 and RelA/p65 subunits, which play critical roles in apoptosis, tumorigenesis, various autoimmune diseases and inflammation. Validated applications for NF-kappa-B (p65) monoclonal antibody are immunofluorescence, Western blotting and immunoprecipitation.

Applications: validated for immunofluorescence, Western blotting and immunoprecipitation
Host species and isotype: mouse IgG1
Clone ID of monoclonal antibody (mAb): 572
Reactivity: human NF-kappa-B (p65). [Based on amino acid sequence homology, reactivity with Rhesus monkey, chimpanzee, rat, dog, cow, horse and mouse, is also expected.]
Product size: 100 µg pack size

Nuclear factor-kB proteins are transcription factors that consist of homo- and heterodimers of NFκB1/p50 and RelA/p65 subunits, which play critical roles in apoptosis, tumorigenesis, various autoimmune diseases and inflammation. In most cell types NFκB is present in the cytoplasm as a 50 kDa (p50) subunit and a 65 (p65, rel A) subunit. Both p50 and 65 share extensive N-terminal homology with the v rel oncogene product. Activation of NFκB is induced by a large number of stimuli and it is tightly regulated by IkB. After cell stimulation IkB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway allowing nuclear translocation of the active dimeric NFκB transcription factor, which is normally present in the cytoplasm. Immunopositivity of active NFκB (p65) has been shown in Hodgkin's and Reed-Sternberg cells. NFκB Is thought to be an important transcriptional regulator for HIV as well as a key to a number of diseases such as Alzheimer's, atherosclerosis and cancer.