The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination.
• Enables individual oligonucleotide design for real-time PCR applications.
• Supports assays based on TaqMan® and SYBR® Green I dye chemistries.
• Provides design flexibility, ease-of-use, and requires minimal optimization.
• Provides robust assay performance when used in accordance with Rapid Assay Development Guidelines (RADG).
The Primer Express® Software v3.0.1 is flexible, easy to learn and to use, and requires minimal optimization. It includes an algorithm optimized specifically for use with TaqMan®-based reagents, recommended primer⁄probe concentrations, and default thermal cycling conditions. Primer Express® Software also supports assays based on the SYBR® Green I dye. This minimizes the need for assay optimization and streamlines the design process.
The software also allows customized designs if the probe and⁄or one or more primers are known. Default and adjustable parameters (Figure 1) provide the flexibility required for all users, regard
Simple to Use
Simplicity and flexibility are the hallmarks of Primer Express® Software v3.0.1, which is fully compatible with all StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems. Whether your research includes gene quantitation or allelic discrimination studies, Primer Express® Software belongs in your lab.
Automated and Manual Primer⁄Probe Design
The Primer Express® Software v3.0.1 will design your primers and probes automatically, or you can design them manually. For automated design, simply choose either TaqMan ® or TaqMan® MGB assays. If you know the sequence, you need only to import (or copy and paste) the sequence and quickly view your results. Alternatively, if you prefer to design the primers and probes manually, the procedure is equally simple and straight-forward as well.
Rapid Assay Development Guidelines
The Primer Express ® Software v3.0.1 was designed with Life Technologies® Rapid Assay Development Guidelines (RADG). The RADG are a simple yet comprehensive set of guidelines for use with our real-time PCR instruments and reagents. Many traditional variables, including magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, which substantially reduces assay development time.
For Research Use Only. Not for use in diagnostics procedures.