The AmpFLSTR® Yfiler® PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.
Key Features of the AmpFLSTR® Yfiler® PCR Amplification Kit:
• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR® kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler® kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)
Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR® Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler® kit can routinely detect a minor male contributor present at less than 1:1000.
Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR® Yfiler® PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler® kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.
A Powerful Tool for Suspect Casework
The Yfiler® kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler® kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.
Complete Validated Solution
Applied Biosystems® offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® kits, instrumentation, software, and consumables. The Yfiler® kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
Note: For Forensic or Paternity Use Only.
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.