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Need Results Fast Applied Biosystems® TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. • Everything you need for TaqMan®-based qPCR amplification and detection in a convenient single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR • TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expression assays • The optimized formulation contains AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup • No AmpErase® UNG, protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination • TaqMan® Fast Universal PCR Master Mix Master(2x), No AmpErase® UNG contains a proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR–related fluorescence fluctuations, for superb precision on Applied Biosystems® real-time PCR instruments
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