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Mouse anti-GCTM2; Antibody specific to Human and Baboon GCTM2. This antibody is validated for Western Blotting, IP, ICC, IHC, and Flow Cytometry. Anti-GCTM2 recognizes an epitope on a 200 kDa protein on the surface of embryonal carcinoma cells. Human embryonal carcinoma cell lysates were used to inject mice and generate monoclonal antibodies. Four different hybridomas were characterized. GCTM-1 stained the nuclei of all human cells tested. GCTM-2 recognized an epitope on a 200 kDa extracellular protein present on the surface of embryonal carcinoma cells, and stained the surface of visceral yolk sac-type carcinoma and colorectal carcinoma cells. Further analysis revealed that the antigen was a keratin sulphate proteoglycan. Two additional hybridomas, GCTM-3 and GCTM-4, were also generated from this fusion having distinctive staining patterns. GCTM-2 also stains tissue sections from human non-seminomatous germ cell tumours, and from seminomas. Fetal gut epithelium and muscle display strong staining in normal human tissues. This antibody is also useful in studying other species, for example by staining baboon kidney sections. GCTM-2 constitutes one of the major reagents for studying embryonal stem cells in varying applications.
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