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This product will be discontinued in April 2014. Alternative products are available—learn more.Close
Perform high-throughput real-time RT-PCR in a single step with the TaqMan® One-Step RT-PCR Master Mix Reagents from Life Technologies. This TaqMan® One-Step RT-PCR Master Mix Reagents Kit (10-pack) combines reverse transcription and cDNA amplification steps into one reaction tube, simplifying your real-time PCR experiments. The kit is ideal for all high-throughput applications, because setup is quick, easy, and eliminates the risk of sample crossover and contamination.
This TaqMan® One-Step RT-PCR Master Mix Reagent Kit includes:
• AmpliTaq Gold® DNA Polymerase: A chemically modified hot-start DNA polymerase that prevents non-specific amplification while providing maximum efficiency • MultiScribe™ Reverse Transcriptase: Provides longer cDNA and higher yield • RNase Inhibitor: Prevents degradation of RNA templates during the reverse transcription step • Passive Reference I: Normalizes signal and corrects inter-well signal variation
Everything in One Kit: Each 10-pack includes 10 TaqMan® One-Step RT-PCR Master Mix Reagents Kits, each of which includes one tube of MultiScribe™ Reverse Transcriptase/RNase Inhibitor Mix (40X) for reverse transcription, and one tube of TaqMan® Universal PCR Master Mix, No AmpErase® UNG (2X) for DNA amplification. Both tubes are pre-mixed for minimal reagent handling and convenience. Just add total or poly-(A)+ RNA, TaqMan® probes, and gene-specific primers for both reverse transcription and amplification. That's all there is to it.
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