Applied Biosystems® AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation.
• Automated chemically modified hot-start enzyme for increased sensitivity, specificity, and yield
• Time-release thermal activation improves low copy target amplification
• Packaged with GeneAmp® 10X PCR Gold Buffer for quick activation, easier optimization, and definitive results
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-release fashion during the denaturation steps of thermal cycling for more difficult PCR applications such as low copy number detection or multiplexed PCR. GeneAmp® 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp® 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold® DNA Polymerase.
AmpliTaq Gold® 360 DNA Polymerase for Superior Performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.