Perform high-throughput real-time RT-PCR in a single step with the TaqMan® One-Step RT-PCR Master Mix Reagents from Life Technologies. The TaqMan® One-Step RT-PCR Master Mix Reagents Kit combines reverse transcription and cDNA amplification steps into one reaction tube, simplifying your real-time PCR experiments. The kit is ideal for all high-throughput applications, because setup is quick, easy, and eliminates the risk of sample crossover and contamination.

This TaqMan® One-Step RT-PCR Master Mix Reagent Kit includes:
• AmpliTaq Gold® DNA Polymerase, a chemically modified hot-start DNA polymerase, prevents non-specific amplification, while providing maximum efficiency
• MultiScribe™ Reverse Transcriptase—provides longer cDNA and higher yield
• RNase Inhibitor—prevents degradation of RNA templates during the reverse transcription step
• Passive Reference I—normalizes signal and corrects inter-well signal variation