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The Applied Biosystems® GeneAmp® Gold RNA PCR Reagent Kit has been optimized to combine reverse transcription using MultiScribe™ Reverse Transcriptase, with highly sensitive and specific amplification using AmpliTaq Gold® DNA Polymerase.
• A two-enzyme/one-buffer system can perform either a one- or two-step reaction chemistry, offering ease of use and flexibility • AmpliTaq Gold®DNA Polymerase automates the hot-start PCR process, ensuring high specificity, high sensitivity, and increased yield • Can be used for multiplexing, saving both time and reagents
One-Step PCR AmpliTaq Gold®DNA Polymerase introduces automatic hot-start to RNA PCR. This kit is specifically designed to perform one-step RNA PCR. It also has the capability to perform two-step RNA PCR better than other available kits.
Improved Performance: The GeneAmp®Gold RNA PCR Reagent Kit allows expression of multiple targets in the same reaction (multiplexing). It also simplifies the use of random hexamers and oligonucleotide d(T) from a long and complicated two-step reaction to one easy step. This kit can be used to produce cDNAs up to a length of 5 kb, and can generate products from as few as 500 copies of known starting material. The GeneAmp® Gold RNA PCR Reagent Kit consists of two components: a core kit that contains all the necessary reagents for 100 RNA PCR amplifications, and a control kit containing control RNA and control primers. The two components are also available separately.
Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.
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